Fig. 1

ZmGPDH1 encodes a cytosol-targeted protein with GPDH activities. a Subcellular localization of ZmGPDH1. Confocal microscopy observation of pBI121-ZmGPDH1::GFP or pBI121-GFP in transgenic Arabidopsis mesophyll protoplasts. Bars = 10 μm. b Coomassie-stained 12% SDS-PAGE of cell extract from (Lane 1) Rosetta (DE3) Escherichia coli strain and (Lane 2–5) DE3 expressing His-tagged ZmGPDH1 protein. c Coomassie-stained 12% SDS-PAGE of (Lane 1) purified ZmGPDH1 fusion protein. d Western blot analysis of (Lane 1) purified ZmGPDH1 fusion protein using anti-6 × His antibody as probe. e The kinetic properties of ZmGPDH1 with regard to DHAP. f GPDH enzyme activities from purified ZmGPDH1 fusion protein. The reaction was performed in the presence (+) and absence (−) of NADH and DHAP, respectively