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Table 7 Primers used for validation of Ganoderma boninense DNA of oil palm roots

From: Transciptome profiling at early infection of Elaeis guineensis by Ganoderma boninense provides novel insights on fungal transition from biotrophic to necrotrophic phase

Primer ID

Accession No.

Sense sequence (5′-3′)

Antisense sequence (5′-3′)

C (control)

KM015454.1

CAACGGATCTCTTGGCTCTC

GCCGATCAATAAAAGACCGA

T1D3 (3 d.p.i)

KM015454.1

CAACGGATCTCTTGGCTCTC

GCCGATCAATAAAAGACCGA

T1D7n (7 d.p.i)

–

GATCGGCTCCTCTCAAATGC

CGGTTAGAAGCTCGCCAAAC

T1D11n (11 d.p.i)

–

GATCGGCTCCTCTCAAATGC

CGGTTAGAAGCTCGCCAAAC

+VE

–

GATCGGCTCCTCTCAAATGC

CGGTTAGAAGCTCGCCAAAC

  1. Note: C - primer for nested PCR from untreated control sample
  2. T1D3 - primer for normal PCR from 3 day-post-inoculation sample
  3. T1D7n* - primer for nested PCR from 7 day-post-inoculation sample
  4. T1D11n* - primer for nested PCR from 11 day-post-inoculation sample
  5. +ve -primer for normal PCR from Ganoderma boninense PER71 pure culture
  6. * retrieved from sequenced PCR amplicon of T1D3 sample
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BMC Plant Biology

ISSN: 1471-2229

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