Fig. 6

Photosynthetic pigments, chlorophyll (a) and carotenoids (b), content referred in mg of pigment per gram of initial fresh weight. Starch content (c) measured as grams of transformed starch per 100 g of fresh weight. Total protein content (d) referred in mg of total soluble protein per gram of initial fresh weight. Proteolytic activities with specific substrates to be degraded by cathepsin L/F-like (e) and B-like (f). HvIcy2 silencing (KD Icy2: 1318, 1322, 1390, 1399 lines) and wild type (WT) barley plants were grown in soil under continuous darkness or 16 h/8 h photoperiod for 14 days. Data are means ± standard error of at least three independent analyses. Different letters indicate significant differences between lines. (P < 0.05, one-way ANOVA followed by Student Newman-Keuls test)