Fig. 1

mRNA expression levels of barley HvIcy-2 gene (a) after 12 (light grey) and 24 h (dark grey) of elicitor treatments in leaves with chitosan (Chit), flagellin (Flg22), and controls (Ctrol), and (b) barley wild type plants after M. oryzae infection at three (3 d) or seven (7 d) days after treatment from leaves of infected (dark grey) or non-infected (light grey) plants. Data were determined by RT-qPCR and expressed as relative mRNA levels of HvIcy-2 gene normalized to barley cyclophilin mRNA content. Data are means ± standard error of at least three independent analyses. Different letters indicate significant differences (P < 0.05, One-Way ANOVA Student Newman-Keuls test)