Fig. 4

Validation of gene expression levels by qRT-PCR. The expression levels of KASI  (a), KASII (b), FATA (c), FATB (d), SAD1 (e), SAD2 (f), FAD2 (g) and FAD8 (h) in the leaves (L), flowers (F) and developing fruits (20, 52, 81, 96, 126 and 146 DAF) were validated with qRT-PCR. ACT11 was selected as the reference gene. Values are means ± SD (normalized that the expression levels in the leaves equal to 1) of three biological replications