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Table 2 Calculations and definitions of fluorescence phenotypes (Strasser et al. 2000) with categorization, broad sense heritability (H2) and p-values of genotype (G), environment (E) and genotype by environment interaction (GxE) effects on fluorescence phenotypes

From: Identification of genomic loci associated with 21chlorophyll fluorescence phenotypes by genome-wide association analysis in soybean

 

Phenotype category

Phenotype

Calculation

Definition

H2 (%)

G

E

GxE

1

Primary fluorescence phenotypes

F0

 

Minimum Fluorescence

36.8

<.0001

<.0001

<.0001

2

FJ

 

Fluorescence intensity at the J-step (2 ms)

29.5

<.0001

<.0001

<.0001

3

FI

 

Fluorescence intensity at the I-step (60 ms)

22.8

<.0001

<.0001

<.0001

4

FM

 

Maximum fluorescence

20.6

<.0001

<.0001

<.0001

5

FV

FM − F0

Variable fluorescence

17.3

<.0001

<.0001

<.0001

6

VJ

(FJ − F0)/(FM − F0)

Variable Fluorescence at the J-step

16.3

<.0001

<.0001

<.0001

7

VI

(FI − F0)/(FM − F0)

Variable fluorescence at the I-step

14.6

<.0001

<.0001

<.0001

8

Relative phenotypes

FM/F0

  

20.7

<.0001

<.0001

<.0001

9

FV/F0

(FM − F0)/F0

Maximum efficiency of photochemistry

20.8

<.0001

<.0001

<.0001

10

FV/FM

(FM − F0)/FM

Maximum yield of primary photochemistry

23.3

<.0001

<.0001

<.0001

11

Extracted phenotypes

M0

4(F300μs − F0)/(FM − F0)

Rate of reaction center closure

23.4

<.0001

<.0001

<.0001

12

N

(Area/(FM − F0))x M0 x(1/VJ)

turn-over number QA reduction events between time 0 and Fm

2.4

0.0002

<.0001

0.0194

13

φPo

1 − (F0/FM)

Maximum yield of primary photochemistry (Fv/Fm)

4.6

<.0001

<.0001

<.0001

14

ψ0

1 − VJ

Likelihood that a trapped exciton can move an electron further than QA−

2.5

<.0001

<.0001

<.0001

15

φEo

(1 − (F0/FM))x Ψ0

Quantum yield of electron transport

2.6

<.0001

<.0001

<.0001

16

φDo

1 − φPo

Quantum yield at time 0 for energy dissipation

4.6

<.0001

<.0001

<.0001

17

PIABS

\( \left(\frac{RC}{ABS}\right)x\left(\frac{\varphi_{Po}}{1-{\varphi}_{Po}}\right)x\left(\frac{\varPsi_0}{1-{\varPsi}_0}\right) \)

Performance Index of PSII normalized for equal absorption

3.2

<.0001

<.0001

<.0001

18

Energy flux phenotypes

ABS/RC

M0x(1/VJ)x(1/φPo)

Energy absorption by antenna per reaction center

6.2

<.0001

<.0001

<.0001

19

TR0/RC

M0x(1/VJ)

Flux of excitons trapped per reaction center

4.7

<.0001

<.0001

0.1487

20

ET0/RC

M0x(1/VJ)x Ψ0

Energy flux for electron transport per reaction center

0.4

<.0001

<.0001

<.0001

21

DI0/RC

(ABS/RC) − (TRo/RC)

Flux ratio of energy dissipation per reaction center

4.7

<.0001

<.0001

<.0001