Fig. 4

Intrinsically disordered character of CdDHN4-L and CdDHN4-S. a Purified protein were detected by SDS-PAGE and stained with Coomassie brilliant blue and after were transferred to PVDF membranes and probed with the anti-K segment of DHNs antibody. b CD spectra of CdDHN4-L and -S were recorded at the same protein concentration (0.25 mg mL^− 1) in deionized water at pH 7.5 and 25 °C. The CD of two proteins difference is not due to differences in protein concentrations or buffer contaminants after protein purification. c FTIR analysis of CdDHN4-L and -S at room temperature with (1 mg powder)