Fig. 2
From: MiR393 and miR390 synergistically regulate lateral root growth in rice under different conditions
Response of miR390/OsARF3 and miR393/OsTIR1 to auxin in rice. a Time course analysis of expression of OsmiR390 and OsARF3 in response to AUX/IAA (auxin/indole-3-acetic acid). 2-week-old seedlings were exposed to 10 μM IAA for designed time. Expression was analyzed by qRT-PCR. *, Significant difference at P < 0.05 compared with No treatment by Student’s t-test (n = 3; means ± SDs). b Time course analysis of expression of OsmiR393a, OsmiR393b and OsTIR1 in response to AUX/IAA (auxin/indole-3-acetic acid). 2-week-old seedlings were exposed to 10 μM IAA for designed time. Expression was analyzed by qRT-PCR. (n = 3; means ± SDs). c GUS staining assay of response of OsmiR390 and OsmiR393 to IAA. GUS staining assay of primary root in MIR390::GUS and MIR393a::GUS was performed after being exposed to 10 μM IAA for 12 h. GUS staining is pointed by white arrows. d Relative GUS activity of MIR390::GUS and MIR393a::GUS in response to IAA. ***, P < 0.001; Student’s t-test