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Fig. 4 | BMC Plant Biology

Fig. 4

From: Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing

Fig. 4

Transcription analysis of selected BY-2 calli transformed with pER8-IR-PIN3. RT-qPCR analysis of transcript levels in eleven randomly selected calli and an untransformed BY-2 callus as a control. a The level of the PIN3 mRNA (RT: oligo dT primer, qPCR: PIN3_F and PIN3_R primers); b the level of the sense intron of the IR-PIN3 (representing both the unspliced transcript and the spliced intron; RT: R5 primer, qPCR: F5 and R5 primers)

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BMC Plant Biology

ISSN: 1471-2229

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