Fig. 8
From: OsPHR3 affects the traits governing nitrogen homeostasis in rice
Effects of total N, NO3− and expression of NRTs and AMTs are Pi-independent in osphr3. Seeds of the WT and mutants (osphr3–1 and 3–2) were grown hydroponically in IRRI solution for 2 weeks and then transferred to +P and -P for (a, b) 2 weeks and (c) 3 d. Shoot and root were harvested for assaying the (a) total N and (b) NO3− concentration. c qRT-PCR was employed for determining the relative expression levels of genes encoding NO3− (OsNRT1.1a, OsNRT2.3a and OsNRT2.4) and NH4+ (OsAMT1.1, OsAMT1.2 and OsAMT1.3) transporters in the root. Actin was used as an internal control and the values for +P WT and + P mutants were normalized to 1. Values of are means ±SE (n = 5) and different letters on the histograms indicate that the values differ significantly (P < 0.05, one-way ANOVA)