The rare orange-red colored Euphorbia pulcherrima cultivar ‘Harvest Orange’ shows a nonsense mutation in a flavonoid 3’-hydroxylase allele expressed in the bracts

Table 2 Characterisation of DFR from enzyme preparations of cv. ‘Christmas Feeling’ bracts

Substrate	DHK	DHQ	DHM
pH optimum	6.50	6.25	5.75
Temperature optimum	40 °C	40 °C	40 °C
Time linearity [min]	20	20	20
Protein linearity [μg in assay]	2.6	0.2	0.2
Apparent k_cat [μmol/kg*s]	97	78	3105
Apparent K_m [μM]	14	0.9	29
k_cat/K_m [l/s*kg]	7	86	107

ISSN: 1471-2229