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Table 2 Characterisation of DFR from enzyme preparations of cv. ‘Christmas Feeling’ bracts

From: The rare orange-red colored Euphorbia pulcherrima cultivar ‘Harvest Orange’ shows a nonsense mutation in a flavonoid 3’-hydroxylase allele expressed in the bracts

Substrate DHK DHQ DHM
pH optimum 6.50 6.25 5.75
Temperature optimum 40 °C 40 °C 40 °C
Time linearity [min] 20 20 20
Protein linearity [μg in assay] 2.6 0.2 0.2
Apparent kcat [μmol/kg*s] 97 78 3105
Apparent Km [μM] 14 0.9 29
kcat/Km [l/s*kg] 7 86 107