Fig. 6

The abundance of a selection of 12 stress-responsive miRNAs assayed using qRT-PCR. a-f Relative abundance of the miRNAs in JN177 plants exposed to stress. g-i Relative abundance of the miRNAs in SR3/SR4 plants compared to JN177 plants exposed to salinity stress. j-l Relative abundance of the miRNAs in SR3/SR4 plants compared to JN177 plants exposed to alkalinity stress. The left hand histogram shows abundances as estimated from miRNA sequencing, and the right hand one as estimated by qRT-PCR. The latter data are given in the form mean ± s.d. (n = 3). CK: non-stressed conditions, NaCl: salinity stress conditions, pH 8.9: alkalinity stress conditions. *, **: means differed significantly at, respectively, P < 0.05 and < 0.01, as determined by the Student’s t-test