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Fig. 6 | BMC Plant Biology

Fig. 6

From: Genome-wide analysis of genes encoding core components of the ubiquitin system in soybean (Glycine max) reveals a potential role for ubiquitination in host immunity against soybean cyst nematode

Fig. 6

Examination of the enzymatic activity of soybean E2s by thioester assay in the presence of soybean E1, GmUBA1. Immunoblot with anti-FLAG antibody was performed following thioester formation assay. Recombinant soybean E2 (GmUBC) proteins were incubated in the presence of GmUBA1 and ubiquitin. Each reactions was split and treated with 100 mM DTT (+) or 4 M urea (-). The numbers at the right indicate the molecular masses of marker proteins in kilo-Dalton (kD)

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BMC Plant Biology

ISSN: 1471-2229

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