Fig. 5
From: Phosphorylation of Arabidopsis SINA2 by CDKG1 affects its ubiquitin ligase activity
Molecular characterization of 35S:CDKG1 and cdkg1 plants. a Schematic diagram of T-DNA insertion in cdkg1–1 and cdkg1–2 mutants and PCR validation of cdkg1–1 and cdkg1–2 mutants (see Additional file 1 Table S1 for primer sequences). Box denotes the single exon of the CDKG1 gene. The positions of the T-DNA inserts are indicated by the triangles and the qPCR primers sites are indicated by the arrows. b RT-qPCR analysis of CDKG1 transcript expression in cdkg1, WT and 35S:CDKG1 lines. AtACTIN2 was used as an internal standard. Data represent the average of three independent assays ± SD with three technical repeats each