Fig. 1

Molecular characterization of sina2 mutants and comparative analysis of their sensitivity to ABA and abiotic stress. a Schematic diagram of T-DNA insertions and PCR-validation of sina2–3 and sina2–1 mutants (primer sequences are shown in Additional file 1 Table S1). Boxes and lines denote exons and introns, respectively. The positions of the T-DNA inserts are indicated by triangles and primer positions are indicated by arrows. b RT-PCR analysis of SINA2 transcript expression in wild type (WT), sina2–3, and sina2–1 lines. AtACTIN2 was used as an internal standard. c Phenotype analysis of sina2 mutant seeds sown on growth media without amendment or containing 300 mM mannitol, 150 mM NaCl, or 0.3 μM ABA. d Quantitative analysis of germination and post-germinative cotyledon greening on 300 mM mannitol, 150 mM NaCl, or 0.3 μM ABA. The percentage of germinated seeds was scored 4 days after stratification and cotyledon greening data was collected 7 days after germination. Each data point represents a mean of 60 seeds ± SD. Asterisks indicate significant differences from WT by student’s t-test (P < 0.01)