Fig. 3
From: An efficient and improved method for virus-induced gene silencing in sorghum
![Fig. 3](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12870-018-1344-z/MediaObjects/12870_2018_1344_Fig3_HTML.png)
Ubiquitin silencing is a better marker for VIGS in sorghum. Sorghum plants were rub inoculated with BMV VIGS constructs to silence ChlH, PDS, or Ubiq genes. a Only BMV::anti-Ubiq inoculated plants had a visible phenotype that is different than the control (BMV::GFP). Each viral vector was rub inoculated on 10 sorghum seedlings. Similar results were observed in all the seedlings. A representative image is shown here. b The RNA was extracted from the second leaf above inoculated leaf of sorghum for gene expression analysis using qRT-PCR. The expression of PDS, ChlH, and Ubiq was significantly lower in the BMV::anti-PDS, BMV::anti-ChlH, and BMV::anti-Ubiq, respectively, compared to control plants (BMV::GFP). Error bars indicate standard deviation of three replicates. The sample was analyzed by student’s t test. Asterisks indicate a statistically significant difference compared with the control at P ≤ 0.01