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Fig. 5 | BMC Plant Biology

Fig. 5

From: Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Fig. 5

Histology of embryonal masses from tertiary lines of the genotype SD4. a, b / SD4–8-1; c,d / SD4–8-2; e,f / SD4–8-3. a, b – Polyembryogenic centers (PECs) Arrowheads in b points to small SEs; c – PECs, smaller ones with distinct embryonal heads; d – detail of PEC; e – PECs, arrowheads points to small SEs; f – detail of PEC with well-organized embryonal heads; m – meristem of PEC, s – suspensor. Paraffin sections stained with Alcian Blue/Nuclear Fast Red. Scale bar: a, b, c = 200 μm; d, F = 100 μm; e = 500 μm

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