Fig. 4

Effect of nitric oxide inhibitor supply on wheat plants under iron deficiency. Harvests were performed at 4, 72 and 96 h of treatment, which corresponded to the three columns per treatment (a) Phytosiderophores (PS) release by Fe-sufficient (+Fe), Fe-starved (-Fe), and Fe-starved plants treated with the NO-scavenger c-PTIO (50 μM); in μM (assuming a Fe-PS chelation 1:1) per g of root fresh weight. Average of 3 replicates with 20 plants each. p < 0.05 (Fisher LSD test). b Relative root TaNAAT gene expression in Fe-sufficient (+Fe), Fe-starved (-Fe), and Fe-starved plants treated with the NO-scavenger c-PTIO (50 μM). Average of 3 replicates with 20 plants each. p < 0.05 (Fisher LSD test). (c) Relative root TaFer1 gene expression in Fe-sufficient (+Fe), Fe-starved (-Fe), and Fe-starved plants treated with the NO-scavenger c-PTIO (50 μM). Average of 3 replicates with 20 plants each. p < 0.05 (Tukey HSD test)