Fig. 5

Comparison of RNA sequencing and qRT-PCR analysis of three differentially expressed genes in (a) chilled flower buds (compared to nonchilled flower buds) (b) and late-pink buds (compared to chilled flower buds). Eukaryotic translation initiation factor 3 subunit H is the internal control. Log₂fold-change was calculated by -∆∆Ct = − [(Ct_GOI – Ct_nom) _{tissue 1} – (Ct_GOI – Ct_nom) _{tissue 2}]. Average Log₂fold-change ± standard deviation of three biological replicates. Significant average fold-change determined using a Student’s t-test is denoted. An asterisk (*) indicates p < 0.001