Fig. 5
From: Dissection of MAPK signaling specificity through protein engineering in a developmental context
Loops A and B are required for specific and robust FAMA stage MKK7 activities. a–f Paired micrographs of representative major phenotype (left) and subcellular localization (right), of specific MKK7ED-YFP variants diagramed above. Black brackets mark clusters and asterisks indicate inhibition. YFP is in green and cell outlines in magenta; white arrowheads point to mitochondrial localization. For example, in (a) MKK7ED induces stomatal clustering (brackets) and is mitochondrial localized (white arrowheads). Scale bars are 50 μm for phenotype images and 10 μm for localization images. g Quantification of phenotypes in (a–f). h SPCH stage inhibition of lineage initiation. Error bars in (g) and (h) correspond to 95% confidence interval. i In vitro kinase activity towards kinase inactive MPK3 and MPK6. Kinase assays were performed in triplicates, normalized to unphosphorylated KI-MPK and averaged; error bars represent standard errors