Fig. 2

Function analysis of GmMAX1a expressed in Arabidopsis. Complementation of GmMAX1a in atmax1–1 mutant and overexpression in the wild-type Col-0 were done to compare the functions of GmMAX1a with AtMAX1. a The seedlings of atmax1–1, Col-0, and atmax1–1 complementation plant with GmMAX1a (atmax1/GmMAX1a) with different primary root lengths (left panel). Quantification of shoot height was conducted and calculated on at least three independent lines of more than 20 plants (right panel). b Mature plants of Col-0, atmax1–1 mutant, complementation (atmax1/GmMAX1a), and GmMAX1a overexpression lines (left panel). The numbers of branches were determined and calculated on at least three independent lines of more than 20 plants (right panel). c The differences between seedlings (top panel) and leaf shapes (bottom panel) of Col-0, atmax1–1, complementation of (atmax1–1/GmMAX1a) and GmMAX1a-OE plants (left panel). Ratios of leaf length to leaf width were collected on 2-week-old seedlings (right panel). Data represent means ± s.d (n = 20 plants for each genotyping). d Silique lengths of Col-0, atmax1–1, and complementation of (atmax1/GmMAX1a) plants (left panel). Quantification of silique length (cm) of Col-0, atmax1–1, complementation of (atmax1/GmMAX1a) and GmMAX1a-OE plants (right panel). Data represent means ± s.d (n = 20 plants for each genotyping). Data are expressed as means ± s.d. from 3 independent experiments with biological replicates. Asterisks indicate significant difference according to a Student’s t-test (**P < 0.001, * P < 0.05)