Fig. 7

Heat maps of orthologues differentially regulated between host and nonhost interactions in wheat and barley. For Blumeria, Magnaporthe and Puccinia interactions, wheat probes with differential expression between host and nonhost interaction were filtered for assignment to barley orthologues differentially expressed in the same pathosystem. On the median-centered normalized signal intensities (c: control, h: host interaction, n: nonhost interaction), a hierarchical clustering (Pearson correlation, average linkage) was performed with the software Genesis [88]. The median-centered signal intensities of barley orthologues were sorted accordingly and illustrated in a heat map. Wheat genes that showed significant higher expression in the nonhost compared to the host interaction at one time point, and were assigned to a barley orthologue with a significant higher expression in nonhost compared to host interaction, are marked in red. Over- or underrepresentation of functional MapMan categories and statistical significance according to Fisher Exact Test were calculated with MapMan ORA tool [35, 36]. All probes assigned to orthologues in the other species were taken as reference. The log ratio of presentation in the gene subset and in the reference is shown for BINs found to be significant (BINs comprising 5 or less probes were excluded). PS, photosynthesis; OPP, oxidative pentose phosphate pathway; TCA/org, tricarboxylic acid cycle/organic acid transformation; misc.: miscellaneous