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Table 2 Number of embryos per explant (NE) for each cultivar after two (T2) and three (T3) months of culture, and the average values. Starting explants consist of 100 seeds distributed a rate of 10 seeds per Petri dish

From: Somatic embryogenesis from seeds in a broad range of Vitis vinifera L. varieties: rescue of true-to-type virus-free plants

Cultivar

Medium

NE at T2

NE at T3

NE/explant

Airen

EIM2

1.70 ± 0.53 b

1.02 ± 0.30 d

1.36 ± 0.26 cd

EIM2/2

0.83 ± 0.60 b

1.21 ± 0.10 cd

1.02 ± 0.41 d

Cabernet Franc

EIM2

1.24 ± 0.62 b

1.61 ± 0.57 cd

1.43 ± 0.58 cd

EIM2/2

2.51 ± 0.25 b

3.16 ± 0.21 bc

2.84 ± 0.24 cd

Cabernet Sauvignon

EIM2

1.39 ± 0.39 b

1.51 ± 0.14 cd

1.45 ± 0.23 cd

EIM2/2

1.14 ± 0.41 b

1.25 ± 0.23 cd

1.19 ± 0.27 cd

Mencía

EIM2

1.34 ± 0.38 b

1.15 ± 0.15 cd

1.24 ± 0.22 cd

EIM2/2

2.03 ± 0.25 b

1.12 ± 0.25 cd

1.57 ± 0.25 cd

Merlot

EIM2

1.57 ± 0.49 b

1.10 ± 0.65 cd

1.34 ± 0.51 cd

EIM2/2

2.19 ± 0.34 b

1.99 ± 0.35 cd

2.09 ± 0.32 cd

Monastrell

EIM2

2.75 ± 0.39 b

2.71 ± 0.34 bcd

2.73 ± 0.24 cd

EIM2/2

2.17 ± 0.58 b

1.25 ± 0.64 cd

1.71 ± 0.61 cd

Petit Verdot

EIM2

2.14 ± 0.27 b

4.15 ± 0.47 b

3.15 ± 0.41 bc

EIM2/2

1.32 ± 0.40 b

1.79 ± 0.63 cd

1.55 ± 0.55 cd

Pinot Blanc

EIM2

2.05 ± 0.19 b

1.40 ± 0.59 cd

1.73 ± 0.32 cd

EIM2/2

1.19 ± 0.35 b

2.24 ± 0.30 bcd

1.72 ± 0.32 cd

Pinot Gris

EIM2

1.35 ± 0.12 b

1.12 ± 0.14 cd

1.24 ± 0.12 cd

EIM2/2

1.20 ± 0.28 b

1.07 ± 0.20 d

1.14 ± 0.26 cd

Pinot Meunier

EIM2

1.78 ± 0.31 b

1.81 ± 0.61 cd

1.79 ± 0.43 cd

EIM2/2

1.15 ± 0.38 b

1.83 ± 0.66 cd

1.49 ± 0.42 cd

Pinot Noir

EIM2

1.35 ± 0.26 b

1.42 ± 0.35 cd

1.39 ± 0.29 cd

EIM2/2

1.19 ± 0.22 b

1.36 ± 0.58 cd

1.27 ± 0.36 cd

Syrah

EIM2

1.00 ± 0.25 b

0.94 ± 0.27 d

0.97 ± 0.21 d

EIM2/2

1.21 ± 0.13 b

1.35 ± 0.45 cd

1.28 ± 0.19 cd

Tempranillo

EIM2

2.20 ± 0.42 b

1.23 ± 0.36 cd

1.71 ± 0.31 cd

EIM2/2

2.12 ± 0.49 b

2.15 ± 0.44 bcd

2.14 ± 0.36 cd

Verdil

EIM2

10.77 ± 0.55 a

10.32 ± 0.25 a

10.54 ± 0.33 a

EIM2/2

8.72 ± 0.58 a

1.50 ± 0.25 cd

5.11 ± 0.53 b

ANOVA

Cultivar

Culture medium Interaction

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*

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*

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  1. Different lower case letters within a column indicate significantly different values (P value < 0.05)
  2. *: P value <0.05
  3. ***: P value <0.001