Fig. 1From: An optimized protocol for the preparation of oxygen-evolving thylakoid membranes from Cyclotella meneghiniana provides a tool for the investigation of diatom plastidic electron transport a Linear gradient from 2 to 3.3 M sorbitol, (b) brightfield (upper panel) and fluorescence (lower panel) microscopic images of the fraction representing the isolated thylakoids of C. meneghinana (both images show the same section, bar represents 2 μm). 2 mL of the FrenchPress extract (approx. 0.8–1 mg Chl mL−1) were loaded onto the sorbitol gradient. For further details concerning the centrifugation step see the Methods sectionBack to article page