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Table 2 M. quinquemaculata gene primers used in various experiments

From: Plant-mediated RNAi silences midgut-expressed genes in congeneric lepidopteran insects in nature

No

Gene

Primer sequences (5′-3′)

Use

1

MqUbiquitin

For- CAAGAAGCGCAAGAAGAAGAAC

Internal control for M. quinquemaculata transcript quantification

Rev- CGTCCACCTTGTAGAACCTAAG

2

MqBG1

For- CCAACCGCCTATGCTGATAAA

Transcript quantification and testing the silencing efficiency of M. quinquemaculata BG1

Rev- GTGACCATGGGTTGGATGTT

3

MqBG2

For- GCTGTATGTTACGGCCAAGA

Transcript quantification and testing the co-silencing efficiency of M. quinquemaculata BG2

Rev- CACGCGCCTTCTACTTGATA

4

MqCYP6B46

For- GTGCCTATTACTCCGCGATCTA

Transcript quantification and testing the silencing efficiency of M. quinquemaculata CYP6B46

Rev- CAAGCCTTCTTTGCTAAACTCC

5

MqCYP6B45

For- GAAATGGATAAATTGGTTTTGACC

Transcript quantification and testing the co-silencing efficiency of M. quinquemaculata CYP6B45

Rev- TTATTTTGACAGAGAAGATTGAGG

6

MqUbiquitin

For- CGACTACAACATCCAGAAGGAG

Amplification of partial coding sequence of M. quinquemaculata Ubiquitin

Rev- GGCTTACGGCTACATCTTAGTC

7

MqBG1

For- GAAGTTGTTGATGCTCGCC

Amplification of partial coding sequence of M. quinquemaculata BG1

Rev- GTGACCATGGGTTGGATG

8

MqBG2

For- GCTGTATGTTACGGCCAAG

Amplification of partial coding sequence of M. quinquemaculata BG2

Rev- GGCTGAATATTGTATTTAAGC

9

MqCYP6B46

For- TGCCTATTACTCCGCGATCTA

Amplification of partial coding sequence of M. quinquemaculata CYP6B46

Rev- TCAATTCGCTTGCGTAGGT

10

MqCYP6B45

For- GATCAAAGATTTCGACGTGTTCAT

Amplification of partial coding sequence of M. quinquemaculata CYP6B45

Rev- ACTTTGAGAGGGAAGATTGAAA