From: Plant-mediated RNAi silences midgut-expressed genes in congeneric lepidopteran insects in nature
No | Gene | Primer sequences (5′-3′) | Use |
---|---|---|---|
1 | MqUbiquitin | For- CAAGAAGCGCAAGAAGAAGAAC | Internal control for M. quinquemaculata transcript quantification |
Rev- CGTCCACCTTGTAGAACCTAAG | |||
2 | MqBG1 | For- CCAACCGCCTATGCTGATAAA | Transcript quantification and testing the silencing efficiency of M. quinquemaculata BG1 |
Rev- GTGACCATGGGTTGGATGTT | |||
3 | MqBG2 | For- GCTGTATGTTACGGCCAAGA | Transcript quantification and testing the co-silencing efficiency of M. quinquemaculata BG2 |
Rev- CACGCGCCTTCTACTTGATA | |||
4 | MqCYP6B46 | For- GTGCCTATTACTCCGCGATCTA | Transcript quantification and testing the silencing efficiency of M. quinquemaculata CYP6B46 |
Rev- CAAGCCTTCTTTGCTAAACTCC | |||
5 | MqCYP6B45 | For- GAAATGGATAAATTGGTTTTGACC | Transcript quantification and testing the co-silencing efficiency of M. quinquemaculata CYP6B45 |
Rev- TTATTTTGACAGAGAAGATTGAGG | |||
6 | MqUbiquitin | For- CGACTACAACATCCAGAAGGAG | Amplification of partial coding sequence of M. quinquemaculata Ubiquitin |
Rev- GGCTTACGGCTACATCTTAGTC | |||
7 | MqBG1 | For- GAAGTTGTTGATGCTCGCC | Amplification of partial coding sequence of M. quinquemaculata BG1 |
Rev- GTGACCATGGGTTGGATG | |||
8 | MqBG2 | For- GCTGTATGTTACGGCCAAG | Amplification of partial coding sequence of M. quinquemaculata BG2 |
Rev- GGCTGAATATTGTATTTAAGC | |||
9 | MqCYP6B46 | For- TGCCTATTACTCCGCGATCTA | Amplification of partial coding sequence of M. quinquemaculata CYP6B46 |
Rev- TCAATTCGCTTGCGTAGGT | |||
10 | MqCYP6B45 | For- GATCAAAGATTTCGACGTGTTCAT | Amplification of partial coding sequence of M. quinquemaculata CYP6B45 |
Rev- ACTTTGAGAGGGAAGATTGAAA |