Catalytic characteristics and isozyme patterns of GLO in vivo.
a GLO enzyme activities in different transgenic plants. OX-GLO1, OX-GLO3, OX-GLO4 and OX-GLO5 represent the GLO1, GLO3, GLO4 and GLO5 overexpression transgenic plants, respectively. Cas9-GLO1 and Cas9-GLO4 represent the GLO1 and GLO4 knockout plants, respectively. Ri-GLO3 and Ri-GLO5 represent the specific GLO3 and GLO5 RNA-silencing transgenic plants, respectively. b The GLO isozyme bands of WT, Cas9-GLO4 and Cas9-GLO1 plants. c The GLO isozyme bands of WT, Ri-GLO3 and Ri-GLO5 plants. The second leaf from the top was detached from plants at 4-leaf stage for determination. Values are means ± SD (n = 3)