Fig. 8

ABA induces positive stomatal modulation in 35S:GaMYB85-overexpressing lines. a The stomatal pores of 35S:GaMYB85 and WT were photographed following treatment with 0, 5, and 10 μM ABA. Five views from three replicate plants were observed at ×40 magnification b Quantitative comparisons of the stomatal pores of 35S:GaMYB85 and WT plants following treatment with 0, 5, and 10 μM ABA. The data shown are the mean values of three replicates ± SD (n = 10). Scale bars: approximately 1 μm c Leaf stomatal density of 35S:GaMYB85 and WT plants photographed under an OLYMPUS Bx51 microscope at ×40 magnification. d Quantitative comparisons of 35S:GaMYB85 and WT stomatal density e Width and length of guard cells measured using ImageJ software. Measurements were taken from the leaves of three plants (10 stomata from five microscopic views). Data are the means ± SD. *P < 0.05; ** P < 0.01