Table 3 Primer sequences
Primer name | Sequence (5′-3′) | Used for |
|---|---|---|
CsERF004-F | ATGGCTCGTCCACAACAACG | Cloning |
CsERF004-R | TTATGTAATAATTTCGAATGATCCGAG | Cloning |
qCsERF004-F | CAACACGTCCACAAACAACA | qRT-PCR |
qCsERF004-R | TGGAATAGACCCACGTTTCA | qRT-PCR |
qCsPR1-F | TGGTGCACTCCAATGGCTCT | qRT-PCR |
qCsPR1-R | GGGTGTAATGGCGACACTCG | qRT-PCR |
qCsPR4-F | ACTGCTTTCTGTGGCCCAGTT | qRT-PCR |
qCsPR4-R | ACCCTCCGTTGGAGCATTGA | qRT-PCR |
qCsEF1a-F | CCAAGGCAAGGTACGATGAAA | qRT-PCR |
qCsEF1a-R | AGAGATGGGAACGAAGGGGAT | qRT-PCR |
qCsEF1a-R | AGAGATGGGAACGAAGGGGAT | qRT-PCR |
GFP-CsERF004-F | GGATCCATGGCTCGTCCACAACAACG | Subcellular localization |
GFP-CsERF004-R | GGACCCGGGTTGTAATAATTTCGAATGATCC | Subcellular localization |