Functional characterization of cucumber (Cucumis sativus L.) Clade V MLO genes

BMC Plant Biology

Table 1 Cuttings of five out of eight CsaMLO1 transformants and two out of seven CsaMLO11 transformants were found to be susceptible to Oidium neolycopersici

Gene	Transformant	Transgene expression	PM symptoms
CsaMLO1	35S::CsaMLO1-A	2.3	+
	35S::CsaMLO1-B	1.3	+
	35S::CsaMLO1-C	1.2	+
	35S::CsaMLO1-D	0.8	−
	35S::CsaMLO1-E	0.7	−
	35S::CsaMLO1-F	0.4	−
	35S::CsaMLO1-G	0.4	+
	35S::CsaMLO1-H	0.1	+
CsaMLO11	35S::CsaMLO11-A	0.3	−
	35S::CsaMLO11-B	0.3	−
	35S::CsaMLO11-C	0.2	+
	35S::CsaMLO11-D	0.2	−
	35S::CsaMLO11-E	0.1	−
	35S::CsaMLO11-F	0.1	−
	35S::CsaMLO11-G	0.1	+

The tomato Slmlo1 mutant, with a frameshift deletion in the SlMLO1 gene [11], was transformed with either a 35S::CsaMLO1 construct or a 35S::CsaMLO11 construct. CsaMLO1 or CsaMLO11 expression in each of the primary transformants (one sample per individual transformant) was quantified relatively to housekeeping gene SlEF-α using qRT-PCR. Two cuttings per individual transformant were inoculated with Oidium neolycopersici, the causal agent of powdery mildew (PM) in tomato. Disease phenotypes were scored based on whether or not PM symptoms were visible on the leaves at 10 days post inoculation

ISSN: 1471-2229