Fig. 6

Fold changes in target gene expression in roots of 360 g ha⁻¹ ethephon-treated black carrot plants relative to untreated plants (dashed horizontal line) at 22 (a) and 25 (b) weeks after sowing. Threshold cycles (Ct) for target genes are standardised to the DcActin2 Ct (ΔCt). The relative expression of target genes in the different harvests is determined according to the 2^(−ΔΔCt) method. Expression levels of target genes in untreated carrots were assigned an arbitrary value of 1. Data represent mean ± SE, n = 3