Fig. 4

Compositional analysis of the CFML. a Cross sections of 10 dpa cotton fibres from PimaS7, FM966 and JFW15 varieties. The de-esterified HG LM19 epitope and the arabinan LM6 epitope are part of the CFML. In contrast, the linear LM13 arabinan epitope labelled all cell walls but was not detected in the enlarged regions and bulges of the CFML. b Calcofluor staining and immunofluorescence detection of the linear arabinan LM13 epitope in cross sections of 17 dpa FM966 and JFW15 fibres. Absence of this epitope was observed in the cell wall area around the centre region of two neighbouring cell walls (arrows), this area consistently corresponded to cell wall regions between paired CFML bulges in the FM966. Scale bars =10 μm. All images in a panel are at the same magnification