Fig. 3
From: Single amino acid substitutions in the selectivity filter render NbXIP1;1α aquaporin water permeable
Expression of N-terminally His-tagged NbXIP1;1α mutants in P. pastoris. Western blots showing the expression levels of N-terminally His-tagged NbXIP1;1 mutants in P. pastoris X-33 clones. Blots were developed on photographic films by enhanced chemiluminiscence. See Additional file 2: Figure S1 for the estimation of protein amounts. a First set of NbXIP1;1α mutants. NbXIP1;1αst is an N-terminally truncated construct of NbXIP1;1α with a fully deleted N-terminal region used as positive control for the western blot. b Second set of NbXIP1;1α mutants