Fig. 6

qRT-PCR analysis of GhmiR157 and GhSPL expression patterns. a Northern blot analysis of mature GhmiR157 expression during floral organ bud developmental stages. b Northern blot analysis of mature GhmiR157 expression in different tissues. R, H, L, P, An, St, 0O, 3O, 5 F, 5O represent the root, hypocotyl, leaf, petal, anther, stigma, 0 DPA ovule, 3 DPA ovule, 5 DPA fibre, and 5 DPA ovule, respectively. c-m Quantitative analysis of GhSPL expression in different floral bud developmental stages. R.E.L., the relative expression levels calculated using HISTONE3 (AF024716) as a control. The error bars indicate the standard deviation of three biological replicates. Different letters in the plots indicate statistically significant differences at P < 0.05 based on analysis of variance (ANOVA) (Tukey’s multiple comparison test). n Images of longitudinal sections of different length (mm) of floral buds and opened flowers. The numbers 0–2, 2–4, 4–6, 6–8, 8–10, 10–16, 16–25 represent different lengths (mm) of floral buds