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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of PaNAC03, a stress induced NAC gene family transcription factor in Norway spruce leads to reduced flavonol biosynthesis and aberrant embryo development

Fig. 4

PaNAC03 overexpression lines lack normal protoderm and display a disturbed maturation response. Embryonal mass formation per gram of proliferating tissue, the asterisk indicate significantly differences in embryonal mass formation between control (P <0.05 t-test) (a). Photos taken after six and eight weeks (b) on maturation medium for the overexpression lines PaNAC03_4.1 and PaNAC03_4.2, expressing PaNAC03 at equal levels (1.7 times the WT), and the wild type represented by the pMDC32-GUS transformed WT line 95:61:21. Scale bar corresponds to 2 mm. Black arrowheads indicate developing embryos. Germination (open bars) and epicotyl growth (grey bars) one and two months after transfer to germination medium (c) in WT and the two OE lines, superscript letters indicate significant differences between treatments (One-way ANOVA, Tukey’s post test)

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