Fig. 4

Clubroot susceptibility in lines defective for fermentation-related genes. a Impact of mutations adh1, pdc1 and pdc2 on the development of clubroot symptoms (eH isolate). b Impact of mutations on the number of spores of P. brassicae per plant at 21 dpi. Data are means of 4 biological repetitions (>12 plant per repetition). Ga/La clubroot disease index is an estimation of the ratio between gall and leaf rosette size from image analysis, as described in Materials and Methods. Bars represent standard errors. Stars represent statistically significant differences between conditions (student T-test, p < 0.05). The number of large spores (>3 μm) was determined using flow cytometry as described in the material & methods section