Fig. 2

Clubroot-induced regulation of the ADH1 promoter visualized through GUS staining. a-c GUS stained upper part of pivotal roots sampled at 21 dpi. a non-inoculated plant b eH isolate c e2 isolate. Rosette leaves were cut after staining for a better visualization of galls. Inserts show the GUS coloration in the lowest part of the root system only in non-inoculated plants. d roots infected with eH (21 pi) cut with a razor blade to show the GUS coloration in root cortical tissues e microscope observation of GUS coloration in a 3 μm slice of infected root (21 dpi). The arrow indicates the vascular structures. f-h GUS stained roots sampled at 7 dpi. f non-inoculated plants g eH isolate, and h GUS induction in non-inoculated plants subjected to a 24-h flooding treatment (positive control). Scale bars indicate 4 mm in a, b and c, 100 μm in e, and 0.5 mm in f-h