Fig. 8
From: Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat
Expression analysis of Psy1 and its homoeologs in developing grains of three genotypes in each F2 population. a M090158. b M090950. c M091949. d M090122. e M091151. f M01217. For each genotype, five biological repeats were sampled and pooled for RNA extraction and gene expression analysis. Transcript levels are given as expression levels relative to the values of wild-type genotypes at 28 DPA (set to 1) after normalization to β-actin level. Data are presented as means ± standard error from three technical replicates. Significant differences (Student’s t test) between homozygous and heterozygous mutant individuals and wild-type genotypes in each F2 population are represented by one or two asterisks: * P <0.05, ** P <0.01. Hom, homozygous mutants; Het, heterozygous mutants; WT, wild-type genotypes