Fig. 2
Genes translationally upregulated under heat stress have much lower propensity to form secondary structure in the vicinity of the start codon. a Average folding energy of translationally upregulated (red line) and downregulated (blue line) genes under heat stress compared to all expressed protein-coding genes. Only in the marked area (inset) the curves shows significant difference (p = 2.2*10−16 (median averaged from a two-sample Mann-Whitney test)). The thin lines in the same color denote the standard deviation for each position. Position 0 is the first nucleotide of the start codon of each genes. b Box plot of the distribution of the folding energy of the genes translationally up- or down-regulated under heat stress compared to all expressed genes. The region −100 nt upstream of the start till the start codon (position 0) is considered. c Sequence motif analysis of 5′UTRs (left logo) and 3′ UTRs (right logo) of genes translationally upregulated under heat stress