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Table 2 Genotype of T1 plants from candidate T0 events harboring mutations within FAD3A

From: Direct stacking of sequence-specific nuclease-induced mutations to produce high oleic and low linolenic soybean oil

Parent line (T0) T1 plant number FAD3A genotype Presence of transgene
Gm183-4 1 −7 bp/WT Undetected
2 −7 bp/-7 bp +
3 −7 bp/-7 bp +
4 −7 bp/WT +
Gm183-5 2 −43 bp/WT +
3 WT/WT Undetected
4 −43 bp/-43 bp +
5 −43 bp/-43 bp Undetected
7 −43 bp/-43 bp +
8 −4 bp/-4 bp +
9 −43 bp/-43 bp +
Gm183-6 1 −4 bp/-4 bp +
2 WT/WT +
3 WT/WT +
4 −4 bp/WT +
5 −4 bp/-4 bp +
6 WT/WT Undetected
7 −4 bp/WT +
8 WT/WT Undetected
9 WT/WT +
10 WT/WT +
11 −4 bp/WT +
12 −4 bp/WT +
13 WT/WT +
14 −4 bp/WT +