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Table 2 Peak data for WUS phosphorylation

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

pI Area %: WUS + pI Area %: WUS-AA +
- MPK3 MPK3 + flg - MPK3 MPK3 + flg
5.19 1.05 0.74 1.14 5.19 2.21 2 1.44
5.26 0.02 0.33 1.8 5.27 1.48 0.79 1.4
5.36 0 1.85 4.03 5.38 1.7 0.44 3.21
5.41 5.76 9.14 18.3     
5.49 0 0 24.17 5.46 8.67 6.93 11.52
5.52 31.31 30.63 15.42 5.53 28.07 27.88 33.33
5.61 54.84 52.13 31.53 5.61 51.61 56.59 43.73
5.68 5.98 4.46 3.1 5.68 5.28 4.18 4.47
5.77 1.04 0.7 0.51 5.77 0.98 1.19 0.89
pI Area %: WUS-DD + pI Area %: WUS-Δdock +
- MPK3 MPK3 + flg - MPK3 MPK3 + flg
5.15 7.39 2.26 3.21 4.95 1.04 2.55 2.11
5.23 6.71 3.65 4.71 5.12 3.76 3.35 5.9
5.32 11.19 11.56 15.47 5.19 7.5 7.84 15.88
5.39 29.76 36.32 36.44 5.25 24.48 28.34 33.19
5.45 43.21 42.89 36.92 5.30 57.25 53.18 38.93
5.53 1.49 2.34 2.42 5.36 4.15 3.47 3.29
5.61 0.26 0.98 0.84 5.42 1.81 1.26 0.7
  1. Corresponding visualised peak areas used for calculating area percentages are shown in Additional file 5: Fig. S3