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Table 1 Peak data for ACS6 C-terminal domain phosphorylation

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

pI Area %: GFP:ACS6-C + pI Area %: GFP:ACS6-C-AAA +
- MPK6 MPK6 + flg - MPK6 MPK6 + flg
4.58 2.7 5.46 3.09 4.59 5.13 6.05 3.09
4.75 6.04 7.23 7.19 4.75 7 7.89 7.21
4.82 9.64 7.33 10.02 4.82 11.02 9.04 9.1
4.89 13.35 9.43 21.45 4.88 16.48 18.74 13.1
5.01 1.54 17.04 20.56 5.03 4.85 5.25 4.48
5.11 0 0 11.44 5.15 8.04 7.93 10.2
5.15 7.09 6.32 4.29     
5.28 23.14 20.46 14.36 5.27 25.15 23.1 27.82
5.39 0 4.73 0 5.39 0 6.4 0
5.44 23.08 14.98 1.58 5.45 5.8 0 8.66
5.55 0 0 3.67 5.55 11.24 10.93 10.48
5.68 11.53 7.02 0     
5.74 0 0 2.34 5.73 5.28 4.67 5.86
  1. Data correspond to the electropherograms shown in Fig. 2b, c