Fig. 5

Phenotype of plants. a Pale-green sectors in leaves of T1 plants containing the iDR intermediate following release of selection. Sectors are linked to iDR recombination and provide a visual illustration of the process. b T2 Seedlings from sectoring T1 plants germinated on spectinomycin medium. White aadA-free seedling is arrowed. c A white aadA-free seedling transferred to antibiotic free medium produces new pale-green leaves. d A marker-free T2 plant containing the edited cp genome exhibits a pale-green phenotype compared to (e), a WT plant