Fig. 4From: Distinct expression and function of carotenoid metabolic genes and homoeologs in developing wheat grainsCharacterization of wheat CCD-A1 and CCD-A4 activities in β-carotene producing E. coli cells. pAC-BETA contains all the genes for β-carotene biosynthesis. Total carotenoids extracted from the growth media of E. coli cells co-expressing pAC-BETA and various plasmid constructs were analyzed by HPLC. HPLC elution profiles between 5 and 11 min are shown. Ascending HPLC traces are offset by 12 s for clarity. β-ionone is indicated by arrow. Inset: absorption spectrum of β-iononeBack to article page