Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

Huang, Ying; Ma, Hong-Yu; Huang, Wei; Wang, Feng; Xu, Zhi-Sheng; Xiong, Ai-Sheng

doi:10.1186/s12870-016-0819-z

Table 2 Proteins differentially expressed between resistant and susceptible tomato cultivars

From: Comparative proteomic analysis provides novel insight into the interaction between resistant vs susceptible tomato cultivars and TYLCV infection

^aSpot no.	^bAccession number	^cProtein name	^dZ_T/Z_C	^dJ_T/J_C
Up-regulated proteins
Spot5	gi\|460372520	Oxygen-evolving enhancer protein 1	2.21^* ± 0.44	10.57^* ± 0.52
Spot10	gi\|100380	Ribulose bisphosphate carboxylase activase	3.25^* ± 0.44	5.57^* ± 0.43
Spot41	gi\|350535160	Wound-inducible carboxypeptidase precursor	3.62^* ± 0.30	5.27^* ± 0.22
Spot42	gi\|350535160	Wound-inducible carboxypeptidase precursor	2.72^* ± 0.19	6.05^* ± 0.30
Spot54	gi\|350536267	Subtilisin-like protease precursor	4.17^* ± 0.29	14.46^* ± 0.40
Spot55	gi\|460381101	28 kDa ribonucleoprotein	2.17^* ± 0.70	2.05^* ± 0.25
Spot60	gi\|460399143	Glycine dehydrogenase (decarboxylating)	4.02^* ± 0.25	5.51^* ± 0.19
Spot61	gi\|460399143	Glycine dehydrogenase (decarboxylating)	6.81^* ± 0.38	5.09^* ± 0.21
Spot63	gi\|170458	Threonine deaminase, partial	5.90^* ± 0.09	7.29^* ± 0.29
Spot64	gi\|170458	Threonine deaminase, partial	5.28^* ± 0.62	2.96^* ± 0.28
Spot65	gi\|170458	Threonine deaminase, partial	4.35^* ± 0.03	7.62^* ± 0.14
Spot68	gi\|460389504	Protein disulfide-isomerase-like	3.51^* ± 0.28	3.39^* ± 0.22
Spot69	gi\|460395973	Heat shock protein 83	19.73^* ± 0.24	9.53^* ± 0.23
Spot77	gi\|590715109	Transducin family protein / WD-40 repeat family protein isoform 1	2.87^* ± 0.03	12.76^* ± 0.11
Spot81	gi\|350536897	Cytosolic ascorbate peroxidase 1	6.03^* ± 0.16	5.85^* ± 0.07
Spot85	gi\|251895	Polyphenol oxidase	15.32^* ± 0.10	10.49^* ± 0.42
Spot86	gi\|460397526	Heme-binding protein 2	4.29^* ± 0.25	4.41^* ± 0.18
Down-regulated proteins
Spot1	gi\|407970998	Chlorophyll a-b binding protein 4	0.22^* ± 0.13	0.18^* ± 0.09
Spot3	gi\|460405507	Chlorophyll a-b binding protein 8	0.46^* ± 0.03	0.36^* ± 0.13
Spot8	gi\|723739979	Ribulose bisphosphate carboxylase/oxygenase activase	0.07^* ± 0.05	0.16^* ± 0.01
Spot11	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase	0.04^* ± 0.01	0.01^* ± 0,01
Spot12	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase	0.06^* ± 0.01	0.02^* ± 0.01
Spot13	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase	0.05^* ± 0.01	0.05^* ± 0.01
Spot14	gi\|488453358	Ribulose 1,5-bisphosphate carboxylase	0.09^* ± 0.01	0.04^* ± 0.01
Spot15	gi\|1293000	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit	0.10^* ± 0.05	0.06^* ± 0.01
Spot17	gi\|21069067	Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit	0.02^* ± 0.01	0.09^* ± 0.08
Spot20	gi\|92087012	Ribulose bisphosphate carboxylase large chain OS	0.09^* ± 0.01	0.01^* ± 0.01
Spot21	gi\|92087012	Ribulose bisphosphate carboxylase large chain OS	0.08^* ± 0.01	0.01^* ± 0.01
Spot22	gi\|460375527	Ferredoxin--NADP reductase, leaf-type isozyme	0.42^* ± 0.10	0.14^* ± 0.02
Spot27	gi\|460372959	Peptidyl-prolyl cis-trans isomerase CYP38	0.26^* ± 0.05	0.15^* ± 0.04
Spot28	gi\|469517896	Glyceraldehyde-3-phosphate dehydrogenase	0.46^* ± 0.03	0.44^* ± 0.13
Spot29	gi\|460414390	ATP synthase delta chain	0.38^* ± 0.05	0.11^* ± 0.03
Spot37	gi\|460373820	ATP synthase gamma chain	0.09^* ± 0.01	0.05^* ± 0.01
Spot39	gi\|350535679	Cytosolic NADP-malic enzyme	0.48^* ± 0.07	0.49^* ± 0.20
Spot43	gi\|350534564	26S protease regulatory subunit 6A homolog	0.20^* ± 0.07	0.30^* ± 0.04
Spot46	gi\|460415494	Eukaryotic initiation factor 4A-2	0.35^* ± 0.02	0.37^* ± 0.02
Spot47	gi\|460399092	Eukaryotic initiation factor 4A-2	0.49^* ± 0.22	0.22^* ± 0.05
Spot57	gi\|460404838	Adenosylhomocysteinase	0.21^* ± 0.06	0.45^* ± 0.19
Spot74	gi\|1168191.1	14-3-3 protein 4 OS	0.10^* ± 0.04	0.11^* ± 0.05
Spot75	gi\|3041662	14-3-3 protein 3	0.21^* ± 0.04	0.08^* ± 0.02
Spot76	gi\|460405902	Plasma membrane-associated cation-binding protein 1	0.20^* ± 0.04	0.34^* ± 0.04
Opposite expression proteins
Spot31	gi\|460397188	Thiamine thiazole synthase	2.25^* ± 0.07	0.23^* ± 0.17
Spot33	gi\|723747143	Aconitate hydratase	0.26^* ± 0.02	5.77^* ± 0.85
Spot45	gi\|460400419	Elongation factor G	0.47^* ± 0.01	3.29^* ± 0.39
Spot48	gi\|460391817	Elongation factor TuB	2.45^* ± 0.23	0.34^* ± 0.03
Spot51	gi\|460391351	Cell division cycle protein 48 homolog	0.38^* ± 0.04	5.90^* ± 0.81
Spot62	gi\|460370413	Glycine dehydrogenase	3.33^* ± 0.16	0.07^* ± 0.02
Spot66	gi\|460398434	Cysteine synthase	2.11^* ± 0.22	0.33^* ± 0.04
Spot83	gi\|460373807	Putative lactoylglutathione lyase	0.44^* ± 0.21	2.05^* ± 0.24

^aNumbering corresponds to the 2-DE in Fig. 3
^bAccession number from the NCBI nr database
^cNames of the proteins obtained via the MASCOT software from the NCBI nr database
^dProtein abundance ratio of Treatment/Control tomato cultivars, with each value representing the mean value ± SD of three biological replicates
^∗Indicates significant (more than 2.0-foldor less than 0.50-fold) difference between control and treatment tomato cultivars

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ISSN: 1471-2229

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