Fig. 4

Northern blot analysis of BrmiR156, BrmiR167 and BrmiR159 in seedlings of Tsuda after exposure to dark, blue light or UV-A. 5′-Digoxigenin-labeled DNA oligonucleotide with complementary sequence to miRNA was used as the probe; BrU6 was used as the control. The gray level ratio between miRNAs and BrU6 was used to compare relative expression to normalized reads