Fig. 5
Constitutively expressing TaBASS2 enhances ROS content and ROS scavenging activity. a-c The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 1 or 1.5 mM H2O2. Bar = 1 cm. d Relative root growth of the wild-type and OE plants treated with 0, 1 or 1.5 mM H2O2. e DAB staining of the leaves from four-week-old soil-grown wild-type and OE plants. f The expression levels of AtCAT1 in 12-day-old wild-type and OE seedlings. g The catalase activity in 12-day-old wild-type and OE seedlings. Error bars in (d) represent the standard errors (n = 3), with each replicate comprising 30 seedlings. Error bars in (f, g) represent the standard errors (n = 3), with each replicate comprising at least 12 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result (P < 0.05, t-test). The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control