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Table 5 Proteins with significant variations in abundance in response to recurrent selection for freezing tolerance

From: A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

     Ratio Statistical significance of variation with a two-way ANOVA
     Non-acclimated Cold-acclimated    
Cultivar Spot no. Homology Function TF3/TF0 TF4/TF0 TF4/TF3 TF3/TF0 TF4/TF0 TF4/TF3 Cold acclimation Populations Cold acclimation * populations
Christie 665 26S protease regulatory subunit 6A homolog Protein metabolism −1.5 −1.3 1.1 −1.9 −1.7 1.1 *** ** NS
669 Actin-101-like Signal transduction −1.3 −1.5 −1.2 −1.9 −1.9 1.0 *** ** NS
1328 Triosephosphate isomerase Carbohydrate and energy metabolism 1.1 2.0 1.9 1.2 1.5 1.3 *** *** NS
1609 Eukaryotic translation initiation factor 5A4 Protein metabolism (Transcription and translation) 2.5 2.4 −1.0 1.5 1.6 1.1 *** ** NS
909 Glyceraldehyde-3-phosphate dehydrogenase Carbohydrate and energy metabolism −1.3 1.4 1.8 1.6 1.7 1.0 NS ** **
Endure 1648 KS-dehydrin Response to stress 1.4 1.2 −1.2 3.4 3.8 1.1 *** ** NS
1032 Annexin 1 Signal transduction −1.3 −1.4 −1.1 1.3 1.5 1.2 *** NS **
1684 Glycine-rich RNA-binding protein Signal transduction 1.8 1.3 −1.4 2.0 −1.4 −2.7 *** ** NS
1656 Pathogenesis-related protein 10a Response to stress (Defense, PR protein) 1.5 1.3 −1.1 1.7 1.3 −1.4 *** ** NS
1694 Pathogenesis-related protein 10a Response to stress (Defense, PR protein) 1.2 1.1 −1.2 1.9 1.3 −1.5 *** ** NS
1722 Pathogenesis-related protein 10a Response to stress (Defense, PR protein) 1.4 1.1 −1.3 1.6 1.3 −1.3 *** ** NS
  1. Significant ratios (≥ |1,5|, P < 0.05 in Student's t-test) under cold-acclimated and non-acclimated conditions are indicated in bold. TF0 = original genetic background; TF3 and TF4 = populations produced after three and four cycles of selection for freezing tolerance, respectively. The statistical significance of variation with a two-way ANOVA is also shown. NS, **, ***: not significant, significant difference at P < 0.01 and P < 0.001 in a two-way analysis of variance (ANOVA), respectively