A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.)

BMC Plant Biology

Table 5 Proteins with significant variations in abundance in response to recurrent selection for freezing tolerance

				Ratio						Statistical significance of variation with a two-way ANOVA
				Non-acclimated			Cold-acclimated
Cultivar	Spot no.	Homology	Function	TF3/TF0	TF4/TF0	TF4/TF3	TF3/TF0	TF4/TF0	TF4/TF3	Cold acclimation	Populations	Cold acclimation * populations
Christie	665	26S protease regulatory subunit 6A homolog	Protein metabolism	−1.5	−1.3	1.1	−1.9	−1.7	1.1	***	**	NS
	669	Actin-101-like	Signal transduction	−1.3	−1.5	−1.2	−1.9	−1.9	1.0	***	**	NS
	1328	Triosephosphate isomerase	Carbohydrate and energy metabolism	1.1	2.0	1.9	1.2	1.5	1.3	***	***	NS
	1609	Eukaryotic translation initiation factor 5A4	Protein metabolism (Transcription and translation)	2.5	2.4	−1.0	1.5	1.6	1.1	***	**	NS
	909	Glyceraldehyde-3-phosphate dehydrogenase	Carbohydrate and energy metabolism	−1.3	1.4	1.8	1.6	1.7	1.0	NS	**	**
Endure	1648	KS-dehydrin	Response to stress	1.4	1.2	−1.2	3.4	3.8	1.1	***	**	NS
	1032	Annexin 1	Signal transduction	−1.3	−1.4	−1.1	1.3	1.5	1.2	***	NS	**
	1684	Glycine-rich RNA-binding protein	Signal transduction	1.8	1.3	−1.4	2.0	−1.4	−2.7	***	**	NS
	1656	Pathogenesis-related protein 10a	Response to stress (Defense, PR protein)	1.5	1.3	−1.1	1.7	1.3	−1.4	***	**	NS
	1694	Pathogenesis-related protein 10a	Response to stress (Defense, PR protein)	1.2	1.1	−1.2	1.9	1.3	−1.5	***	**	NS
	1722	Pathogenesis-related protein 10a	Response to stress (Defense, PR protein)	1.4	1.1	−1.3	1.6	1.3	−1.3	***	**	NS

Significant ratios (≥ |1,5|, P < 0.05 in Student's t-test) under cold-acclimated and non-acclimated conditions are indicated in bold. TF0 = original genetic background; TF3 and TF4 = populations produced after three and four cycles of selection for freezing tolerance, respectively. The statistical significance of variation with a two-way ANOVA is also shown. NS, **, ***: not significant, significant difference at P < 0.01 and P < 0.001 in a two-way analysis of variance (ANOVA), respectively

ISSN: 1471-2229