Construction of a high-density genetic map by specific locus amplified fragment sequencing (SLAF-seq) and its application to Quantitative Trait Loci (QTL) analysis for boll weight in upland cotton (Gossypium hirsutum.)

Table 2 The whole process of filtering markers

Filtered step	Number
All the Reads	443.65 MB
The Reads of High Quality with Q20	364.86 MB
SLAFs in the Reads	53,754
SNPs in the SLAFs	160,876
Polymorphic SNPs across the Whole RIL Population	23,519
SNPs of AA × BB Genotype	18,318
Deep of SNPs More Than Four	16,490
Polymorphic SNPs between parents	15,076
Percentage of Missing Data less than 40 %	10,588
SNPs with non segregation distortion (p ≥ 0.05) and with significant segregation distortion (0.001 < P < 0.05)	5521

ISSN: 1471-2229