Fig. 1From: Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinasesMBP Phosphorylation and Thiophosphorylation by MPK3/4/6. a Kinase activity assay of purified wild-type (Wt) and kinase-death (KD) forms of MPK3/4/6 with (+) and without (-) pre-phosphorylation by upstream MKKs. b In vitro thiophosphorylation of MBP by Wt and KD forms of MPK3/4/6 in the presence (+) or absence (-) of ATPγS. CBB: Coomassie Brilliant BlueBack to article page