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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of Vitreoscilla hemoglobin increases waterlogging tolerance in Arabidopsis and maize

Fig. 4

Generation and confirmation of transgenic VHb maize plants. a, Resistant calli. b, Plantlets regenerated from resistant calli. c, Plantlets grown in greenhouse. d, Genotyping for the transgene. PCR reactions were performed on the genomic DNA of putative transgenic maize plants using primers targeting the VHb gene. Lane 1, DL2000 DNA marker. Lane 2, positive control: the pCAMBIA3301-VHb construct. Lane 3, negative control: maize inbred line A188. Lanes 4–10, transgenic plants

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